kinA [2017-08-28 08:24:42]

two-component sensor kinase, phosphorylates [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|Spo0F], part of the [SW|phosphorelay]

locus: BSU13990

pI: 5.49

mw: 68.99 kDa

protein length: 606 aa

gene length: 1818 bp

function: initiation of sporulation

product: two-component sensor kinase

essential: no

synonyms: spoIIF,spoIIJ,gsiC,scoD

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.1|The kinases]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.1|The kinases]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

Gene

Coordinates: 1,470,026 → 1,471,846

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|Spo0F]
• mainly active in the older, inner regions of a colony (with [protein|2111AC1AE49D1006E10DC127BF5B7A0327DE94A7|KinB]) [Pubmed|21097618]

[SW|Domains]

• three tandem [SW|PAS domain]s in the N-terminal region of KinA, the [SW|second PAS] domain is the major N-terminal determinant of KinA dimerization [Pubmed|23504013]
• the first [SW|PAS domain] is required for NAD(⁺) binding [Pubmed|23599347]
• C-terminal histidine phosphotransferase domain

Modification

• autophosphorylation on a His residue

Effectors of protein activity

• interaction with [protein|5A5E17D39296FECC1C03B1A7791AB1065BB4E4DF|PxpB] or [protein|CEFD10FAFA0DBC83CD2B61DAC9339FEE025B1611|Sda] inhibits autophosphorylation of [protein|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|KinA] [Pubmed|9334321,15023339]
• autophosphorylation is inhibited by [protein|E5E2994D96FCECEE75E15136A08308C7B6C1C183|SivA] and [protein|E06A3392E644FEDC6F46ED41BBE89168F59B76B3|BslA] [Pubmed|23335417]
• activated by interaction with NAD(⁺) that indicated poor respiratory activity of the cell [Pubmed|23599347], this has been falsified [pubmed|28449380]

Structure

• [PDB|2VLG] (PAS domain)

[SW|Localization]

• cytoplasm

additional information

• the protein forms tetramers [pubmed|28449380]

Expression and Regulation

Operons

• genes: [gene|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|kinA]
  description: [Pubmed|1569009]

  sigma factors

  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|1569009], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [Pubmed|9644251], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [regulon|stringent response|stringent response]: positive regulation, in [regulon|stringent response|stringent response]

  regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|9644251]

  additional information

  • promoter-up mutations or high artificial expression that render ''[SW|kinA]'' independent from [SW|SigH], allow [SW|sporulation] at high salinity [Pubmed|26712348]

References

Reviews

• 22303284

Original Publications

• 23169620,20511506,19561131,20413551,1846779,11292798,16166384,11734624,9299348,12067336,18174125,10094672,11069677,15023339,2509430,17350039,9334321,9477965,9644251,1569009,20081035,21097618,21926229,22303282,22670053,23335417,23378509,23504013,23599347,25598361,26055117,26165942,26657919,26712348,27216630,28449380,18324779